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anti canine il 6 detection antibody  (R&D Systems)


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    R&D Systems anti canine il 6 detection antibody
    Anti Canine Il 6 Detection Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+serum+albumin+duoset+elisa/Human+Serum+Albumin+DuoSet+ELISA/us12607627-558-31-35
    Average 94 stars, based on 85 article reviews
    anti canine il 6 detection antibody - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Evaluating drug-drug interaction risk associated with peptide analogues using advanced in vitro systems.
    Article Snippet: .. Published on December 1, 2023 as DOI: 10.1124/dmd.123.001441 at A SPE T Journals on D ecem ber 29, 2023 dm d.aspetjournals.org D ow nloaded from 16 measured using the Human Serum Albumin DuoSet ELISA and DuoSet ELISA Ancillary Reagent Kit 2, following the protocol of the manufacture (R&D Systems). ..

    Article Title: Improved functionality of hepatic spheroids cultured in acoustic levitation compared to existing 2D and 3D models.
    Article Snippet: .. Albumin quantification in the supernatants was performed with Human Serum Albumin DuoSet ELISA (RnD Systems™), according to the manufacturer’s instructions. .. The optical density at 450 nm was detected using a microplate reader (VarioskanTMLUX multimode, Thermo ScientificTM, Waltham, MA USA).

    Article Title: Comparative Study between the 3D‐Liver Spheroid Models Developed from HepG2 and Immortalized Hepatocyte‐Like Cells with Primary Hepatic Stellate Coculture for Drug Metabolism Analysis and Anticancer Drug Screening
    Article Snippet: The amount of cancer biomarkers secreted into the culture media was measured by Milliplex Kit circulating cancer biomarkers according to the manufacturer’s protocol (Merck, Germany). .. The quantity of albumin production within the culture media was determined by Human Serum Albumin DuoSet ELISA (R&D system, USA) following manufacturing protocol. ..

    Article Title: Modeling drug-induced liver injury and screening for anti-hepatofibrotic compounds using human PSC-derived organoids
    Article Snippet: .. Levels of human albumin, MCP-1, and IL-6 were measured by the human serum albumin duoset ELISA (R&D system, DY1455), human MCP-1 (CCL2) mini TMB ELISA development kit (Peprotech, 900-TM31), and human IL-6 mini TMB ELISA development kit (Peprotech, 900-TM16), respectively. .. Organoids were fixed with 4% paraformaldehyde (PFA) in PBS (30 min, room temperature), followed by permeabilization in 0.2% Triton X-100 in PBS (1 h, room temperature) and blocking (1% BSA in PBS) for 1 h. Samples were then incubated with primary antibodies against Albumin (1:100, R&D systems, MAB1455), SOX9 (1:500, Millipore, AB5535), PDGFRβ (1:200, Abcam, ab32570), Collagen I (1:500, Abcam, ab34710) at 4 °C for 24 h and secondary antibodies Alexa 488 donkey-anti-rabbit (1:200, Invitrogen, R37118), Alexa 647 donkey-anti-mouse (1:1000, Invitrogen, A21235) for 3 h. Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (1:2000, Beyotime, C1002).

    Article Title: Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice
    Article Snippet: .. Levels of human albumin were determined by the human serum albumin duoset ELISA (R&D system, DY1455) according to the manufacturer’s instructions. .. For immunostainings of albumin and Ki-67 in HLOs, organoids were harvested at the day 24 and fixed with 4% paraformaldehyde for 30 min, followed by permeabilization with 0.1% Triton-X-100 for 1 h and 1% BSA blockage for 1 h. Samples were then incubated with primary antibodies against Albumin (1:100, R&D systems, MAB1455) and Ki-67 (1:300, CST, 12202 S) at 4 °C for 24 h, and the following secondary antibodies Alexa 488 donkey-anti-rabbit (1:200, Invitrogen, R37118), and Alexa 647 goat-anti-mouse (1:1000, Invitrogen, A21235) for 3 h. Nuclei were stained with DAPI (1:2000, Beyotime, C1002).

    Article Title: Improved functionality of hepatic spheroids cultured in acoustic levitation compared to existing 2D and 3D models
    Article Snippet: .. Albumin quantification in the supernatants was performed with Human Serum Albumin DuoSet ELISA (RnD Systems™), according to the manufacturer’s instructions. .. The optical density at 450 nm was detected using a microplate reader (VarioskanTMLUX multimode, Thermo ScientificTM, Waltham, MA USA).

    Article Title: Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice.
    Article Snippet: .. Levels of human albumin were determined by the human serum albumin duoset ELISA (R&D system, DY1455) according to the manufacturer’s instructions. .. For immunostainings of albumin and Ki-67 in HLOs, organoids were harvested at the day 24 and fixed with 4% paraformaldehyde for 30min, followed by permeabilization with 0.1% Triton-X-100 for 1 h and 1% BSA blockage for 1 h. Samples were then incubated with primary antibodies against Albumin (1:100, R&D systems, MAB1455) and Ki-67 (1:300, CST, 12202 S) at 4 °C for 24 h, and the following secondary antibodies Alexa 488 donkey-anti-rabbit (1:200, Invitrogen, R37118), and Alexa 647 goat-anti-mouse (1:1000, Invitrogen, A21235) for 3 h. Nuclei were stained with DAPI (1:2000, Beyotime, C1002).

    Article Title: Engineering a three-dimensional liver steatosis model.
    Article Snippet: Reactions were performed in technical duplicates on the BioRad CFx96 and the fold change was quantified using the delta delta CT method as outlined in Livak et al [37]. .. Media from the 20 organoids per condition (control and steatotic) was analyzed for its albumin content using the Human Serum Albumin DuoSet ELISA (DY1455, RnD Systems) and the DuoSet ELISA Ancillary Reagent Kit 2 (DY008B, RnD Systems). ..

    Control:

    Article Title: Engineering a three-dimensional liver steatosis model.
    Article Snippet: Reactions were performed in technical duplicates on the BioRad CFx96 and the fold change was quantified using the delta delta CT method as outlined in Livak et al [37]. .. Media from the 20 organoids per condition (control and steatotic) was analyzed for its albumin content using the Human Serum Albumin DuoSet ELISA (DY1455, RnD Systems) and the DuoSet ELISA Ancillary Reagent Kit 2 (DY008B, RnD Systems). ..



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